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PublicaciónChemical Composition, Tocopherol and Carotenoid Content of Seeds from Different Andean Lupin (Lupinus mutabilis) Ecotypes(Springer, 2021)Andean lupin (Lupinus mutabilis) seeds are appreciated for their high protein and lipid contents and have potential applications as ingredients in food, cosmetic, and pharmaceutical industries. Nevertheless, the information about the seed composition (especially in lipophilic antioxidants) of ecotypes from distinct cropping areas is currently limited. Thus, the aim of the present research was to assess the morphological characteristics, chemical composition, tocopherol and carotenoid contents of the seeds of 33 Andean lupin ecotypes from different Peruvian regions, along with three L. albus, one L. angustifolius and one L. luteus controls. Significant differences were noted among the Andean ecotypes for all analyzed features. The protein, lipid and ash contents were 32.0–46.9, 13.6–18.6 and 2.7–4.4 g/100 g dry matter (DM), respectively. The seeds were rich in tocopherols (172.1–249.8 mg/kg DM; γ-tocopherol was 98% of total tocols) and low in carotenoids (0.69–2.89 mg/kg DM). Debittering increased the tocopherol content (227.0–378.2 mg/kg DM), probably because of the soluble components loss, although the carotenoid concentration remained unchanged. The Andean lupins had higher protein, lipid and tocopherol contents than L. albus and L. angustifolius; the L. luteus values were within the L. mutabilis range. These results suggest that L. mutabilis harbors nutritional characteristics that are well suited to modern food trends. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.
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PublicaciónCharacterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas](Universidad Nacional Mayor de San Marcos, 2019)The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved.
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PublicaciónThe neotropical genus hapalotremus simon, 1903 (Araneae: Theraphosidae), with the description of seven new species and the highest altitude record for the family(Taylor and Francis Ltd., 2018)New specimens of the genus Hapalotremus (Theraphosinae) are revised based on the examination of types and additional material collected in Argentina, Bolivia and Peru. A new generic diagnosis is proposed. New information and illustrations are provided for known species and seven new species were recognized and are newly described and illustrated. Hence, Hapalotremus comprises 10 valid species, distributed along the Andes and Yungas in western South America. All species are keyed and mapped. New taxonomic features are included in the descriptions and the detail of embolus keels morphology and positions are described for the first time for the genus. Information on species habitat is included. The highest altitude record for a Theraphosidae spider, living at 4524 m above sea level, is reported.
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PublicaciónPhenolic profiles of andean mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers: Identification by HPLC-DAD and evaluation of their antioxidant activity(Elsevier Ltd, 2008)Qualitative high performance liquid chromatography with diode array detection (HPLC-DAD) was performed to characterize nonanthocyanin phenolic compounds in three different coloured mashua genotypes. The ORAC antioxidant activity contribution in the tubers related to the type of phenolic compounds present was also evaluated. Phenolic compounds were analysed by separating them into four main fractions: fraction I obtained by means of a liquid–liquid partition with ethyl acetate and fractions II, III and IV obtained by elution on a Sephadex LH-20 column. Fraction I revealed the presence of gallic acid, gallocatechin, procyanidin B2 and epigallocatechin. Other phenolic compounds such as hydroxycinnamic and hydroxybenzoic acid derivatives, rutin and/or myricetin derivatives were also present in fraction I. Fraction II was mainly composed of epicatechin, hydroxycinnamic and hydroxybenzoic acid derivatives. Fraction III presented mainly anthocyanins for the purple coloured mashua tubers and rutin, hydroxycinnamic acid and hydroxybenzoic acid derivatives for the yellow coloured genotype. Fraction IV was composed of proanthocyanidins. Alkaline and acid hydrolysis of the different fractions revealed the presence of gallocatechin, epicatechin, p-coumaric acid, o-coumaric acid, cinnamic acid, protocatechuic acid, rutin and quercetin as the main phenolic moieties present. The proanthocyanidin fractions were the major contributors to the ORAC antioxidant activity of the mashua tubers for two of the three genotypes (34.7–39.2%). The results obtained in the present study confirm that mashua tubers constitute a promising source of antioxidant phenolics and could potentially be considered as a functional food with beneficial health effects.
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PublicaciónMeasurement of Neutral-Current K+ Production by Neutrinos using MINERvA(American Physical Society, 2017)Neutral-current production of Kþ by atmospheric neutrinos is a background in searches for the proton decay p → Kþν¯. Reactions such as νp → νKþΛ are indistinguishable from proton decays when the decay products of the Λ are below detection threshold. Events with Kþ are identified in MINERvA by reconstructing the timing signature of a Kþ decay at rest. A sample of 201 neutrino-induced neutral-current Kþ events is used to measure differential cross sections with respect to the Kþ kinetic energy, and the nonKþ hadronic visible energy. An excess of events at low hadronic visible energy is observed relative to the prediction of the NEUT event generator. Good agreement is observed with the cross section prediction of the GENIE generator. A search for photons from π0 decay, which would veto a neutral-current Kþ event in a proton decay search, is performed, and a 2σ deficit of detached photons is observed relative to the GENIE prediction.
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