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Chronic hypoxia induces modification of the N-methyl-D-aspartate receptor in rat brain
Chronic hypoxia induces modification of the N-methyl-D-aspartate receptor in rat brain
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Fecha
1996
Autores
Pichiule P.
Chávez J.C.
Boero J.
Arregui A.
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Editor
Elsevier
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Abstracto
This study examined [3H]MK-801 binding to the N-methyl-D-aspartate (NMDA) receptor in membranes prepared from cerebral
cortex, hippocampus and corpus striatum of 3 week old rats exposed to 10 weeks of intermittent hypobaric hypoxia (4300 m; 450 Torr)
and compared results wiLh those of normoxic controls. The cortex, hippocampus and striatum of hypoxic animals had a 36, 35 and 31%
reduction in binding sites (Bmax) and a 29, 32 and 17% decrease (reflecting increased affinity) in the dissociation constant (Kd) when
compared to controls. In the cerebral cortex, both glutamate (100 #M) and glycine (10 #M) enhanced [3H]MK-801 binding by two to 3-
fold: Coagonist glutamate, however, had a higher ECs0 (0.44 #M) in the hypoxic cortical membranes when compared to controls (0.28
#M). No significant differences were found in the ECs0 of glycine. The results show that the NMDA receptor is altered in several brain
regions of rats developing in a hypoxic environment.
Descripción
This work was supported in part by a grant from the Consejo Nacional de Ciencia y Tecnologfa (CONCYTEC) of Perti. (+)-MK-801 was generously supplied by Dr. Leslie Iversen (Merck, Sharpe and Dohme, UK).
Palabras clave
n methyl dextro aspartic acid receptor blocking agent,
dizocilpine,
glutamic acid,
glycine,
n methyl dextro aspartic acid receptor,
animal experiment,
animal model,
animal tissue