3.2 Pasantías y ponencias
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PublicaciónResponses of oral-microflora-exposed dental pulp to capping with a triple antibiotic paste or calcium hydroxide cement in mouse molars(Elsevier BV, 2020)Introduction: Responses of oral-microflora-exposed dental pulp to a triple antibiotic paste (TAP), a mixture of ciprofloxacin, metronidazole, and minocycline in ointment with macrogol and propylene glycol, remain to be fully clarified at the cellular level. This study aimed to elucidate responses of oral-microflora-exposed dental pulp to capping with TAP in mouse molars. Methods: A cavity was prepared on the first molars of 6-week-old mice to expose the dental pulp for 24 h. The exposed pulp was capped with TAP (TAP group) or calcium hydroxide cement (CH group), in addition to the combination of macrogol (M) and propylene glycol (P) (MP, control group), followed by a glass ionomer cement filling. The samples were collected at intervals of 1, 2, and 3 weeks, and immunohistochemistry for nestin and Ki-67 and deoxyuride-5'-triphosphate biotin nick end labeling (TUNEL) assay were performed in addition to quantitative real-time polymerase chain reaction (qRT-PCR) analyses. Results: The highest occurrence rate of pulp necrosis was found in the control group followed by the CH group at Weeks 2 and 3, whereas the highest occurrence rate of healed areas in the dental pulp was observed in the TAP group at each time point. Tertiary dentin formation was first observed in the dental pulp of the TAP group at Week 2. In contrast, bone-like and/or fibrous tissues were frequently observed in the CH group. qRT-PCR analyses clarified that TAP activated the stem and dendritic cells at Weeks 1 and 2, respectively. Conclusions: The use of TAP as a pulp-capping agent improved the healing process of oral-microfloraexposed dental pulp in mouse molars. (C) 2020, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
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PublicaciónA comparative study on the phenolic bioaccessibility, antioxidant and inhibitory effects on carbohydrate-digesting enzymes of maca and mashua powders(Academic Press, 2020)The polyphenolic content of dehydrated maca (MA) and mashua (M) powders, promising functional ingredients, and their antioxidant activity and inhibition of carbohydrate digestion was evaluated following in vitro gastrointestinal digestion (GID). Moreover, the impact of steaming on bioaccessibility and bioactivity of the M phenolic fraction was investigated. MA phenolic fraction composed mainly of caffeic-4-O-glucosides showed high bioaccessibility (117.8%), antioxidant activity (142.9 μmol Trolox equivalents, TE/g) and weak effectiveness to inhibit carbohydrate digestion after GID. Anthocyanins were the major compounds in the M phenolic fraction with low bioaccessibility (22.4%), although a superior antioxidant activity (446.7 μmol TE/g) and modest inhibitory effects on carbohydrate digestion after GID. Generally, bioaccessibility and bioactivity of M decreased after steaming process. MA and M powders contain appreciable concentrations of phenolics, although M polyphenols were more effective on modulating carbohydrate digestion. © 2020 Elsevier Ltd
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PublicaciónEvaluation and selection of biochars and hydrochars derived from agricultural wastes for the use as adsorbent and energy storage materials(Elsevier Ltd, 2021)The utilization of unconventional agricultural wastes to obtain new porous carbonaceous materials, at mild pyrolysis temperatures and without complex procedures, for either water treatment and energy storage applications is important from the economic and environmental perspective. In this study, biochars and hydrochars were prepared from banana rachis, cocoa pod husks, and rice husks at 600 °C-2 h, under nitrogen flux. The prepared materials were characterized to better understand how their morphological, textural, physical-chemical and/or structural properties correlate with their methylene blue (MB) adsorption capacities. The material with the best properties (mainly SBET > 800 m2/g) and MB adsorption capacity was a novel biochar prepared from banana rachis (BW-BC). This novel material was selected for additional kinetics and equilibrium adsorption tests for lead (Pb) along with its energy storage capacity. In equilibrium test, the novel biochar reached a maximum adsorption capacity for methylene blue of 243.4 mg/g and the highest adsorption capacity for Pb(II) of 179.7 mg/g. In the kinetic adsorption test, the equilibrium adsorption value for methylene blue was 150.4 mg/g and that for Pb(II) was 159.6 mg/g. Most importantly, the performance of the BW-BC material for energy storage in supercapacitors surpassed that of the commercial activated carbon YP50F, reaching specific energy values of 6.66 and 8.52 Wh/kg in acidic and neutral electrolytes, respectively. Among the evaluated hydrochar and biochars derived of agrowastes, the biochar prepared from banana rachis showed the best properties, being potentially useful as adsorbent or as an electrode material for energy storage. © 2021 Elsevier Ltd
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PublicaciónComputer Aided Medical Diagnosis Tool to Detect Normal/Abnormal Studies in Digital MR Brain Images(IEEE Xplore, 2014)This work presents a model to support medical diagnosis through the classification of abnormality normality in medical brain images, in order to help to specialist as a previous step in the brain pathology diagnosis. Our proposal was incorporated into a content-based image retrieval system, thus we developed a useful tool for radiologists. The first step produces the features vector of MR image using Gabor Filter for the data train and test, then as second step features vector of training data are indexed into CBIR module. The third step makes the training of SVM and as four step the test dataset is classified with the SVM trained. Finally, the result of classification are presented with a set of similar images product of a KNN query. This model was implemented as a software tool with graphical interface. We obtained 94.12% of correct classification. Our medical image dataset is composed of 187 MRI images collected from a medical diagnosis company and selected by medical specialist. The result shows that the proposed model is robust and effective as a software tool to aid support to medical diagnostic.
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PublicaciónA Comparison of Digital Modelling Techniques Analyzing a Section of Qhapaq Nan(IEEE Xplore, 2015)Total Station has been one of the most common acquisition devices for achieving maps through topographic survey. Nowadays, Terrestrial Laser Scanner (TLS) and Photogrammetry are commonly used to generate accurate meshes. In addition, commercial products such as Kinect offer low cost technology to acquire point-cloud information. The present paper aims to measure the accuracy of these digital modelling techniques by employing elevation contour maps, surface deviations and distance measurements. For this purpose, a 450 m sector of the Qhapaq Nan located in Lima-Peru, was selected as a case of study. A camera-enabled drone was used for acquiring pictures to obtain a high-resolution photogrammetric model. Subsequently, a 3D survey of the monument was conducted with a time-of-flight laser scanner. Contour elevation lines where extracted from TLS, Photogrammetry and Total Station models at the same depths in order to determine the precision of photogrammetry and laser scanner reconstructions. In addition, geometrical comparisons were performed among the 3D models above mentioned and the Kinect sensor. The comparison showed that TLS is the most accurate tool for 3D reconstruction. However, Photogrammetry and Kinect provided errors of less than one centimeter in accuracy.
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