Influence of the soluble-insoluble ratios of cyclodextrins polymers on the viscoelastic properties of injectable chitosan-based hydrogels for biomedical application

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Palomino-Durand C.
Lopez M.
Cazaux F.
Martel B.
Blanchemain N.
Chai F.
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Injectable pre-formed physical hydrogels provide many advantages for biomedical applications. Polyelectrolyte complexes (PEC) formed between cationic chitosan (CHT) and anionic polymers of cyclodextrin (PCD) render a hydrogel of great interest. Given the difference between water-soluble (PCDs) and water-insoluble PCD (PCDi) in the extension of polymerization, the present study aims to explore their impact on the formation and properties of CHT/PCD hydrogel obtained from the variable ratios of PCDi and PCDs in the formulation. Hydrogels CHT/PCDi/PCDs at weight ratios of 3:0:3, 3:1.5:1.5, and 3:3:0 were elaborated in a double⁻syringe system. The chemical composition, microstructure, viscoelastic properties, injectability, and structural integrity of the hydrogels were investigated. The cytotoxicity of the hydrogel was also evaluated by indirect contact with pre-osteoblast cells. Despite having similar shear⁻thinning and self-healing behaviors, the three hydrogels showed a marked difference in their rheological characteristics, injectability, structural stability, etc., depending on their PCDi and PCDs contents. Among the three, all the best above-mentioned properties, in addition to a high cytocompatibility, were found in the hydrogel 3:1.5:1.5. For the first time, we gained a deeper understanding of the role of the PCDi/PCDs in the injectable pre-formed hydrogels (CHT/PCDi/PCDs), which could be further fine-tuned to enhance their performance in biomedical applications.
This research was funded by FONDECYT–CONCYTEC (grant contract number 274-2015-FONDECYT).
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Watersoluble polymers, Chitosan, Cyclodextrins, Functional polymers, Medical applications, Polyelectrolytes, Shear flow, Shear thinning, Stability, Viscoelasticity, Injectable hydrogels, Insoluble polymers, Self-healing, Viscoelastic properties, Hydrogels