Publicación:
Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa
Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa
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Fecha
2018
Autores
Paucar-Menacho, Luz María
Martínez-Villaluenga C.
Dueñas M.
Frias J.
Peñas E.
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Editor
Blackwell Publishing Ltd
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Abstracto
Germination has been proposed as an economic approach to improve the content of bioactive compounds
in pseudocereals. In this work, response surface methodology (RSM) was employed to investigate the
impact of germination conditions on the phytochemical content and antioxidant activity of quinoa. The
use of desirability methodology showed that the optimum conditions to maximise the content of total
phenolic content (TPC) and antioxidant activity in sprouted quinoa were 20 °C for 42 h. Sprouts produced under these conditions exhibited increases of 80% and 30% in TPC and antioxidant activity,
respectively, compared to un-germinated seeds, and contained high c-aminobutyric acid (GABA) concentration. The nonsignificant lack-of-fit and high determination coefficients obtained confirmed the suitability of the predictive models developed for TPC and antioxidant activity, whilst the one obtained for
GABA was not significant (R2 < 0.75) within the conditions studied. Sprouting under optimum conditions
enhanced the content of both flavonoid and nonflavonoid compounds, being the increase in flavonoids
more pronounced. Kaempferol-O-dirhamnosyl-galactopyranose and quercetin-O-glucuronide were the
compounds that experienced the most noticeable increase in quinoa after germination. In conclusion, this
study provides useful information on the optimum germination conditions to improve the levels of
health-promoting compounds in quinoa.
Descripción
Palabras clave
Surface properties,
Amino acids,
Antioxidants,
Cultivation,
Flavonoids,
Phenols,
Aminobutyric acids,
Anti-oxidant activities,
Germination,
Phenolic compounds,
Quinoa,
Response surface methodology,
Plants (botany)