Publicación:
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas
No hay miniatura disponible
Fecha
2019-06-25
Autores
Tarqui Terrones, Kathia
Ramirez Carranza, Giovanna
Beltrán Fabián, Maria
Título de la revista
Revista ISSN
Título del volumen
Editor
Instituto Nacional de Salud (Peru)
Proyectos de investigación
Unidades organizativas
Número de la revista
Abstracto
The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp.
Descripción
Palabras clave
Sedimentación en tubo,
Giardia spp.,
Sacarosa,
Gradiente de densidad,
Recuento de células