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Evaluation of the selective antibacterial activity of Eucalyptus globulus and Pimenta pseudocaryophyllus essential oils individually and in combination on Enterococcus faecalis and Lactobacillus rhamnosus

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dc.contributor.author Ambrosio C.M.S. es_PE
dc.contributor.author de Alencar S.M. es_PE
dc.contributor.author Moreno A.M. es_PE
dc.contributor.author Da Gloria E.M. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2018
dc.description The authors are grateful to the “Ministerio de Educación del Perú” from the Peruvian government for Carmen M.S Ambrosio M.Sc. scholarship, granted by the program “Programa Nacional de Becas y Crédito Educativo” (PRONABEC). Also, the author thanks CIENCIACTIVA for the recent Carmen M.S. Ambrosio Ph.D. scholarship, from the “Consejo Nacional de Ciencia, Tecnología e Inovación Tecnológica” (CONCYTEC-Peru; Contract 278-2015-FONDECYT).
dc.description.abstract Essential oils (EOs), as substitutes for antibiotics in animal diets, should have selective antibacterial activity between pathogenic and beneficial bacteria from the animal gut. Thus, this study evaluated the selective antibacterial activity of Eucalyptus globulus (EG) and Pimenta pseudocaryophyllus (PP) EOs on Enterococcus faecalis as a surrogate model of pathogenic bacterium and on Lactobacillus rhamnosus as a beneficial bacterium model. The EOs antibacterial activity was evaluated by determination of minimal inhibitory concentrations (MICs), minimal bactericidal concentration (MBCs), and fractional inhibitory concentration (FIC) indices. The time-kill and sequential exposure assays were also performed, but using only the EG oil, which was the best selective EO, since it had a MIC lower on E. faecalis (7.4 mg/mL) than on L. rhamnosus (14.8 mg/mL). FIC index values showed that the combination of the two EOs had an indifferent effect (1.25 and 2.03) on E. faecalis and an additive effect (1.00) on L. rhamnosus. The time-kill assay showed that EG oil was able to kill E. faecalis within 15 min of treatment (∼5 log reduction) and caused a reduction ∼3 log of L. rhamnosus viability. The sequential exposure assay showed that EG oil (at MIC/2) produced higher reduction on E. faecalis viability (∼3 log) than on L. rhamnosus (∼2 log) as well. Therefore, L. rhamnosus presented higher tolerance to the antibacterial activity of EG oil than E. faecalis did.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1139/cjm-2018-0021
dc.identifier.scopus 2-s2.0-85055643450
dc.identifier.uri https://hdl.handle.net/20.500.12390/608
dc.language.iso eng
dc.publisher Canadian Science Publishing
dc.relation.ispartof Canadian Journal of Microbiology
dc.rights info:eu-repo/semantics/openAccess
dc.subject myrcene
dc.subject 3 carene es_PE
dc.subject alpha phellandrene es_PE
dc.subject alpha thujene es_PE
dc.subject beta pinene es_PE
dc.subject chavibetol es_PE
dc.subject cineole es_PE
dc.subject essential oil es_PE
dc.subject eugenol es_PE
dc.subject limonene es_PE
dc.subject methyleugenol es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.00.00
dc.title Evaluation of the selective antibacterial activity of Eucalyptus globulus and Pimenta pseudocaryophyllus essential oils individually and in combination on Enterococcus faecalis and Lactobacillus rhamnosus
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
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