Publicación:
Low-melanin containing pullulan production from sugarcane bagasse hydrolysate by Aureobasidium pullulans in fermentations assisted by light-emitting diode

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Fecha
2017
Autores
Ahmed M.A.
da Silva S.S.
dos Santos J.C.
Marcelino P.F.
Menegatti C.R.
Orsi C.A.
Terán Hilares R.
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Elsevier Ltd
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Abstracto
Pullulan is a polymer produced by Aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. In this study, light-emitting diodes (LEDs) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of A. pullulans LB83 with different carbon sources. Under white light using glucose-based medium, 11.75 g.L−1 of pullulan with high melanin content (45.70 UA540nm.g−1) was obtained, this production improved in process assisted by blue LED light, that resulted in 15.77 g.L−1 of pullulan with reduced content of melanin (4.46 UA540nm.g−1). By using sugarcane bagasse (SCB) hydrolysate as carbon source, similar concentration of pullulan (about 20 g.L−1) was achieved using white and blue LED lights, with lower melanin contents in last. Use of LED light was found as a promising approach to assist biotechnological process for low-melanin containing pullulan production.
Descripción
The authors gratefully acknowledge the Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica (CONCyTEC-Perú, process number 219-2014), FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo – Process number 2015/26698-0) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil) for financial support. The authors also gratefully acknowledge to Dr. Fernando Carlos Pagnocca from Center for Study of Social Insects (CEIS/UNESP) for the donation of the strain Aureobasidium pullulans LB 83.
Palabras clave
Melanin pigments, Bagasse, Carbon, Diodes, Fermentation, Melanin, Enzymatic hydrolysates, Exopolysaccharides
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