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Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes

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dc.contributor.author Valdivia M. es_PE
dc.contributor.author Castañeda-Zegarra S. es_PE
dc.contributor.author Lévano G. es_PE
dc.contributor.author Lazo J. es_PE
dc.contributor.author Reyes J. es_PE
dc.contributor.author Bravo Z. es_PE
dc.contributor.author Santiani A. es_PE
dc.contributor.author Mujica F. es_PE
dc.contributor.author Ruíz J. es_PE
dc.contributor.author Gonzales G.F. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2019
dc.description This study was supported by Grant PIBA15A106 and B17100101 Universidad Nacional Mayor de San Marcos and National Council of Science Technology and Innovation N° 313-2011-CONCYTEC-OAJ. This article includes results of Martha Valdivia ?octoral Thesis of Sciences with mention of Physiology, Universidad Peruana Cayetano
dc.description.abstract The identification system of spermatogonial stem cell (SSC) was established in alpaca using the molecular expression as well as the reactivity pattern to Dolichos biflorus agglutinin (DBA) by flow cytometry. Twenty-four testicles with their epididymis were recovered from adult alpacas at the slaughterhouse of Huancavelica—Perú. Samples were transported to the Laboratory of Reproductive Physiology at Universidad Nacional Mayor de San Marcos. Testes were selected for our study when the progressive motility of epididymal spermatozoa (ESPM) was above 30%. Isolation of SSC was performed with two enzymatic digestions. Finally, sperm viability was evaluated by means of the trypan blue vital stain in spermatogonial round cells. Samples with more than 80% viability were selected. Isolated cells cultured for 2 days were used for identifying the presence of SSCs by the expression of integrin β1 (116 bp) and PLZF (206 bp) genes. Spermatogonia were classified according to the DBA reactivity. Spermatogonia with a strong positive to DBA (sDBA+) were classified as SSC (Mean ± SEM=4.44 ± 0.68%). Spermatogonia in early differentiation stages stained weakly positive with DBA (wDBA+) (Mean ± SEM=37.44 ± 3.07%) and differentiated round cells as DBA negative (Mean ± SEM=54.12 ± 3.18%). With the use of molecular and DBA markers, it is possible to identify easily the spermatogonial stem cells in alpaca.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1111/and.13283
dc.identifier.scopus 2-s2.0-85063990103
dc.identifier.uri https://hdl.handle.net/20.500.12390/942
dc.language.iso eng
dc.publisher Blackwell Publishing Ltd
dc.rights info:eu-repo/semantics/openAccess
dc.subject SSC
dc.subject Alpaca es_PE
dc.subject DBA es_PE
dc.subject epididymal sperm mobility es_PE
dc.subject molecular markers es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.03
dc.title Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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