Publicación:
Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama) [Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)]
Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama) [Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)]
| dc.contributor.author | Calderon H.B. | es_PE |
| dc.contributor.author | Alave E.G.C. | es_PE |
| dc.contributor.author | Coronel V.O.Y. | es_PE |
| dc.contributor.author | Rojas C.P. | es_PE |
| dc.contributor.author | Pérez M.G. | es_PE |
| dc.contributor.author | Rey O.A.C. | es_PE |
| dc.contributor.author | Ferreyra C.B. | es_PE |
| dc.contributor.author | Felix B.T. | es_PE |
| dc.contributor.author | Neyra D.G. | es_PE |
| dc.contributor.author | Arellano R.R.I. | es_PE |
| dc.contributor.author | Ormachea S.S. | es_PE |
| dc.contributor.author | Arevalo H.M. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50 ) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 ?g/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 ?L/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice. © 2020, Instituto Nacional de Salud. All rights reserved. | |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.doi | https://doi.org/10.17843/rpmesp.2020.373.4597 | |
| dc.identifier.scopus | 2-s2.0-85096307252 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/2527 | |
| dc.language.iso | eng | |
| dc.publisher | Instituto Nacional de Salud | |
| dc.relation.ispartof | Revista Peruana de Medicina Experimental y Salud Publica | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Neutralization | |
| dc.subject | Bothrops atrox | es_PE |
| dc.subject | Immune Sera | es_PE |
| dc.subject | Lama glama | es_PE |
| dc.subject | Lethal Dose 50 | es_PE |
| dc.subject | Mortality (Source: MeSH NLM) | es_PE |
| dc.subject.ocde | http://purl.org/pe-repo/ocde/ford#3.02.27 | |
| dc.title | Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama) [Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)] | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication |