Publicación:
Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama) [Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)]
Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama) [Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)]
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Fecha
2020
Autores
Calderon H.B.
Alave E.G.C.
Coronel V.O.Y.
Rojas C.P.
Pérez M.G.
Rey O.A.C.
Ferreyra C.B.
Felix B.T.
Neyra D.G.
Arellano R.R.I.
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Instituto Nacional de Salud
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Abstracto
Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50 ) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 ?g/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 ?L/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice. © 2020, Instituto Nacional de Salud. All rights reserved.
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Palabras clave
Neutralization,
Bothrops atrox,
Immune Sera,
Lama glama,
Lethal Dose 50,
Mortality (Source: MeSH NLM)