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Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum

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dc.contributor.author Muñoz-Atienza E. es_PE
dc.contributor.author Távara C. es_PE
dc.contributor.author Díaz-Rosales P. es_PE
dc.contributor.author Llanco L. es_PE
dc.contributor.author Serrano-Martínez E. es_PE
dc.contributor.author Tafalla C. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2019
dc.description The authors want to thank Lucía González and Laura Fernández for technical assistance. Dr. Erin Bromage (University of Massachusetts Dartmouth, USA) is also acknowledged for supplying the anti-IgD and anti-CD3 antibodies used in this study. This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM). Carlos Távara would like to acknowledge the financial support from the Master Program in Aquatic Animal Health of the UPCH subsidized by Cienciactiva of CONCYTEC (Management Agreement No. 230-2015- FONDECYT-DE-PROMOCION 2). Carlos Távara also would like to acknowledge Vitapro S.A. for the interest in this research and support in the development of this work. E. Muñoz-Atienza holds a Juan de la Cierva-Formación Postdoctoral Contract (FJCI-2015-24452) funded by the Spanish Ministry of Economy, Industry and Competitiveness.
dc.description.abstract Flavobacterium psychrophilum is the etiological agent of bacterial cold water disease (BCWD), also referred to as rainbow trout fry syndrome (RTFS), a disease with great economic impact in salmonid aquaculture. Despite this, to date, not many studies have analyzed in depth how the immune system is regulated during the course of the disease. In the current study, we have studied the transcription of several immune genes related to T and B cell activity in the skin of rainbow trout (Oncorhynchus mykiss) naturally infected with F. psychrophilum in a farm located in Lake Titicaca (Peru). The levels of expression of these genes were tested and compared to those obtained in asymptomatic and apparently healthy rainbow trout. In the case of symptomatic fish, skin samples containing characteristic ulcerative lesions were taken, as well as skin samples with no lesions. Our results pointed to a significant local up-regulation of IgD, CD4, CD8, perforin and IFNγ within the ulcerative lesions. On the other hand, no differences between the levels of expression of these genes were visible in the spleen. To confirm these results, the distribution of IgD+ and CD3+ cells was studied through immunohistochemical techniques in the ulcerative lesions. Our results demonstrate a strong local response to F. psychrophilum in rainbow trout in which IgD and T cells seem to play a major role.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1016/j.fsi.2018.11.027
dc.identifier.scopus 2-s2.0-85056652191
dc.identifier.uri https://hdl.handle.net/20.500.12390/677
dc.language.iso eng
dc.publisher Academic Press
dc.relation.ispartof Fish and Shellfish Immunology
dc.rights info:eu-repo/semantics/openAccess
dc.subject gamma interferon
dc.subject CD4 antigen es_PE
dc.subject complementary DNA es_PE
dc.subject DNA 16S es_PE
dc.subject elongation factor 1alpha es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.01.08
dc.title Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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