Publicación:
Magnetic bio-nanocomposite catalysts of CoFe2O4/hydroxyapatite-lipase for enantioselective synthesis provide a framework for enzyme recovery and reuse

dc.contributor.author Saire-Saire S. es_PE
dc.contributor.author Garcia-Segura S. es_PE
dc.contributor.author Luyo C. es_PE
dc.contributor.author Andrade L.H. es_PE
dc.contributor.author Alarcon H. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2020
dc.description.abstract Enzymatic catalysis is a sustainable alternative for cost-prohibitive catalysts based on noble metals and rare earths. Enzymes can catalyze selective reactions under mild conditions. Enzyme recovery after a reaction for its reuse is still a challenge for industrial application. Herein, a biocompatible magnetic nanocomposite is presented as alternative for enzyme stabilization and easy recovery. The magnetic core of CoFe2O4 provides capabilities for magnetic recovery. Two different functionalization methods based on adsorption of enzyme onto biocompatible hydroxyapatite (HAP) and through covalent bonding using a molecular spacer based on 3-Aminopropyl)triethoxysilane (APTES) have been evaluated. Both enzymatic bio-nanocomposites presented high selectivity for the transesterification reaction of racemic mixtures of (R,S)-1-phenylethanol, with complete conversion of (R)-1-phenylethanol enantiomer. Studies with different solvent and temperature had demonstrated high range of operation conditions due to enzyme stabilization provided by surface attachment. Meanwhile, magnetic properties allowed easy recovery through application of an external magnetic field for enzyme reuse. Results showed high stability of lipase covalently bond to CoFe2O4/HAP over several reaction cycles.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1016/j.ijbiomac.2020.01.137
dc.identifier.scopus 2-s2.0-85078059118
dc.identifier.uri https://hdl.handle.net/20.500.12390/2563
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof International Journal of Biological Macromolecules
dc.rights info:eu-repo/semantics/openAccess
dc.subject Nano-biocatalysis
dc.subject Enantioselective synthesis es_PE
dc.subject Enzymatic synthesis es_PE
dc.subject Hydroxypatite es_PE
dc.subject Magnetic nanoparticles es_PE
dc.subject.ocde http://purl.org/pe-repo/ocde/ford#2.10.02
dc.title Magnetic bio-nanocomposite catalysts of CoFe2O4/hydroxyapatite-lipase for enantioselective synthesis provide a framework for enzyme recovery and reuse
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
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