Successive pretreatment and enzymatic saccharification of sugarcane bagasse in a packed bed flow-through column reactor aiming to support biorefineries

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Terán-Hilares R.
Reséndiz A.L.
Martínez R.T.
Silva S.S.
Santos J.C.
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Elsevier Ltd
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A packed bed flow-through column reactor (PBFTCR) was used for pretreatment and subsequent enzymatic hydrolysis of sugarcane bagasse (SCB). Alkaline pretreatment was performed at 70 °C for 4 h with fresh 0.3 M NaOH solution or with liquor recycled from a previous pretreatment batch. Scheffersomyces stipitis NRRL-Y7124 was used for fermentation of sugars released after enzymatic hydrolysis (20 FPU g−1 of dry SCB). The highest results for lignin removal were 61% and 52%, respectively, observed when using fresh NaOH or the first reuse of the liquor. About 50% of cellulosic and 57% of hemicellulosic fractions of pretreated SCBs were enzymatically hydrolyzed and the maximum ethanol production was 23.4 g L−1 (ethanol yield of 0.4 gp gs−1), with near complete consumption of both pentoses and hexoses present in the hydrolysate during the fermentation. PBFTCR as a new alternative for SCB-biorefineries is presented, mainly considering its simple configuration and efficiency for operating with a high solid:liquid ratio.
The authors gratefully acknowledge the Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica (CONCyTEC-Perú, process number 219-2014) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil, process number 449609/2014-6) for financial support.
Palabras clave
xylose, Bagasse, Bioethanol, Ethanol, Fermentation, Hydrolysis, Packed beds, Refining, Saccharification, Bio-ethanol production, Enzymatic saccharification, Packed bed reactor, Reuse of alkaline liquor, Sugar-cane bagasse, Enzymatic hydrolysis, alcohol, bagasse, cellobiose, cellulose, glucose, hemicellulose, hexose, ionic liquid, lignin, pentose, alcohol, bagasse, biofuel, bioreactor, cellulose, enzyme activity, ethanol, fermentation, hydrolysis, lignin, sugar cane, temperature effect, alcohol production, alkalinity, Article, biomass, cell growth, enzyme activity, fermentation, hydrolysis, infrared spectroscopy, liquid, nonhuman, packed bed flow through column reactor, priority journal, reactor, saccharification, scanning electron microscopy, Scheffersomyces stipitis, sugar intake, sugarcane, water content, X ray diffraction, budding yeast, evaluation study, fermentation, metabolism, sugarcane, ultrastructure, Biofuels, Cellulose, Ethanol, Fermentation, Hydrolysis, Lignin, Saccharomycetales, Saccharum