Publicación:
Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS
Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS
| dc.contributor.author | Solis L. | es_PE |
| dc.contributor.author | Coronel J. | es_PE |
| dc.contributor.author | Rueda D. | es_PE |
| dc.contributor.author | Gilman R.H. | es_PE |
| dc.contributor.author | Sheen P. | es_PE |
| dc.contributor.author | Zimic M. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Tuberculosis (TB) control efforts are hampered by a mismatch in diagnostic technology. Lack of adequate early diagnostics and Multi-drug resistant (MDR) detection is a critical problem in control efforts. Alternate and novel diagnostic approaches are required, especially in low-resources settings where they are needed most. The Microscopic Observation Drug Susceptibility (MODS) assay is a cost-effective, highly sensitive, and specific method based on the detection of characteristic cording growth patterns of Mycobacterium tuberculosis (MTB), in microscopic examination of a liquid culture under an inverted microscope. By adding antimicrobials to the wells, MODS also determines antimicrobial susceptibility in both MDR and Extreme Drug Resistant (XDR) tuberculosis. The interpretation of a MODS culture performed in a 24 well plate, requires an extensive inspection over the entire surface to detect TB cords. This process requires significant time and effort from a trained microscopist. We evaluated a lens-free imager system, able to render microscopic images of live specimens, for the proof of principle to be used for MODS culture interpretation. The lens-free imager system is able to digitalize a 24-mm2 surface with approximately 40X magnification in a single capture. The evaluation of the lens-free imager found that it produced microscopic images that were adequate for MODS interpretation by a human expert. Compared to the average time that takes a microscopist to completely examine a MODS culture sample, the lens free imager notably reduced the time of inspection. Therefore, lens-free imager variants may constitute promising systems to aid in the diagnostics of tuberculosis, by simplifying and reducing the time of inspection and permitting automatization of MODS interpretation. | en |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.doi | https://doi.org/10.1016/j.tube.2015.12.001 | |
| dc.identifier.scopus | 2-s2.0-84960448523 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/602 | |
| dc.language.iso | eng | |
| dc.publisher | Churchill Livingstone | |
| dc.relation.ispartof | Tuberculosis | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | tuberculostatic agent | |
| dc.subject | antiinfective agent | es_PE |
| dc.subject | antibiotic sensitivity | es_PE |
| dc.subject | Article | es_PE |
| dc.subject | bacterial growth | es_PE |
| dc.subject | cost effectiveness analysis | es_PE |
| dc.subject | diagnostic imaging equipment | es_PE |
| dc.subject | diagnostic procedure | es_PE |
| dc.subject | drug screening | es_PE |
| dc.subject | drug sensitivity | es_PE |
| dc.subject | human | es_PE |
| dc.title | Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication | |
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