Publicación:
Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS
Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS
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Fecha
2016
Autores
Solis L.
Coronel J.
Rueda D.
Gilman R.H.
Sheen P.
Zimic M.
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Editor
Churchill Livingstone
Proyectos de investigación
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Abstracto
Tuberculosis (TB) control efforts are hampered by a mismatch in diagnostic technology. Lack of adequate
early diagnostics and Multi-drug resistant (MDR) detection is a critical problem in control efforts.
Alternate and novel diagnostic approaches are required, especially in low-resources settings where they
are needed most.
The Microscopic Observation Drug Susceptibility (MODS) assay is a cost-effective, highly sensitive, and
specific method based on the detection of characteristic cording growth patterns of Mycobacterium
tuberculosis (MTB), in microscopic examination of a liquid culture under an inverted microscope. By
adding antimicrobials to the wells, MODS also determines antimicrobial susceptibility in both MDR and
Extreme Drug Resistant (XDR) tuberculosis.
The interpretation of a MODS culture performed in a 24 well plate, requires an extensive inspection
over the entire surface to detect TB cords. This process requires significant time and effort from a trained
microscopist.
We evaluated a lens-free imager system, able to render microscopic images of live specimens, for the
proof of principle to be used for MODS culture interpretation. The lens-free imager system is able to
digitalize a 24-mm2 surface with approximately 40X magnification in a single capture.
The evaluation of the lens-free imager found that it produced microscopic images that were adequate
for MODS interpretation by a human expert. Compared to the average time that takes a microscopist to
completely examine a MODS culture sample, the lens free imager notably reduced the time of inspection.
Therefore, lens-free imager variants may constitute promising systems to aid in the diagnostics of
tuberculosis, by simplifying and reducing the time of inspection and permitting automatization of MODS
interpretation.
Descripción
Palabras clave
tuberculostatic agent,
antiinfective agent,
antibiotic sensitivity,
Article,
bacterial growth,
cost effectiveness analysis,
diagnostic imaging equipment,
diagnostic procedure,
drug screening,
drug sensitivity,
human