Development of a New Electrochemical Sensor Based on Mag-MIP Selective Toward Amoxicillin in Different Samples

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Aguilar-Pineda, Jorge A.
Caracela-Zeballos, Claudia
Chavez-Fumagalli, Miguel A.
Davila Del-Carpio, Gonzalo
Fernandez-Macedo, Victor
Lino Cardenas, Christian L.
Nieto-Montesinos, Rita
Peralta-Mestas, Antero
Pino-Figueroa, Alejandro
Shrivastava, Pallavi
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Frontiers Media SA
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This work describes an electrochemical sensor for the selective recognition and quantification of amoxicillin and a beta-lactam antibiotic in real samples. This sensor consists of a carbon paste electrode (CPE) modified with mag-MIP (magnetic molecularly imprinted polymer), which was prepared by precipitation method via free radical using acrylamide (AAm) as functional monomer, N,N '-methylenebisacrylamide (MBAA) as a crosslinker, and potassium persulfate (KPS) as initiator, to functionalized magnetic nanoparticles. The magnetic non-imprinted polymers (mag-NIP) were prepared using the same experimental procedure without analyte and used for the preparation of a CPE for comparative studies. The morphological, structural, and electrochemical characteristics of the nanostructured material were evaluated using Field emission gun scanning electron microscopy (FEG-SEM), Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), Vibrating sample magnetometry (VSM), X-ray diffraction (XRD), and voltammetric technique. Electrochemical experiments performed by square wave voltammetry show that the mag-MIP/CPE sensor had a better signal response compared to the non-imprinted polymer-modified electrode (mag-NIP/CPE). The sensor showed a linear range from 2.5 to 57 mu mol L-1 of amoxicillin (r(2) = 0.9964), with a limit of detection and a limit of quantification of 0.75 and 2.48 mu mol L-1, respectively. No significant interference in the electrochemical signal of amoxicillin was observed during the testing experiments in real samples (skimmed milk and river water). The proposed mag-MIP/CPE sensor could be used as a good alternative method to confront other techniques to determine amoxicillin in different samples.
This project was supported by FONDECYT-BM (grant #0232019-FONDECYT-BM-IN.INV), FAPESP (grants #2019/006777 and #2014/50945-4), CNPq (grants #301728/2019-4, #408050/2018-7 and #465571/2014-0), and CAPES (PROJ. AUX/PE/PROEX N. 0674/2018). RL and GP received financial support from the OGI, Research Management Bureau (Project FC-F-6-2018).
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selective adsorption