Publicación:
Preliminary safety evaluation of n-butanol from the collagen extraction process and of collagen extract from Oreochromis niloticus (tilapia) skin oriented for dermocosmetics
Preliminary safety evaluation of n-butanol from the collagen extraction process and of collagen extract from Oreochromis niloticus (tilapia) skin oriented for dermocosmetics
Fecha
2021
Autores
Sánchez Tuesta, Linda Cristina
Reátegui Pinedo, Nataly Yahayra
Salirrosas Fernández, Remigio David
Morocho-Jácome, Ana Lucía
Daud Sarruf, Fernanda
Miliani Martinez, Renata
Quevedo-León, Roberto
Fiestas Jacinto, Ramiro
Ayala-Jara, Carmen
Rolim Baby, André
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Associação Lusófona para o Desenvolvimento da investigação e Ensino em Ciências da Saúde (ALIES)
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Abstracto
Obtaining new cosmetic ingredients, mainly from sustainable sources, as novel excipients or even active compounds is noteworthy for the cosmetic industry to enhance new innovative dermocosmetics. Thus, it is essential to establish the safety of these new ingredients to avoid adverse events, mainly those associated with clastogenic effects from the chemical compounds used for collagen extraction. In this study, we evaluated solutions of chemical compounds used in the collagen extraction process from tilapia skin (Oreochromis niloticus). The cytotoxic and genotoxic effects of the solutions used in the collagen extraction process were 10.0, 1.0, 0.5, and 0.1% n-butanol. Solutions were evaluated by the Allium test and the comet assay in peripheral white blood cells. The residual water from the final skin wash in the pre-treatment phase and the 0.5% lyophilized collagen extract were also investigated. The absence of cytotoxic and genotoxic activity was demonstrated in the collagen extract, despite the fact that n-butanol showed DNA damage, both in the root cells of Allium cepa and in the white blood cells of human peripheral blood. Therefore, we note the necessity to carry out genotoxicity tests to guarantee the absence of contaminants in the collagen extract for cosmetic purposes.
Descripción
Authors are very thankful to CONCYTECFONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-BM-IADT/AV), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Process 305250/2019-1, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) Finance Code 001 and Programa Nacional de Pós-Doutorado (PNPD).
Palabras clave
Oreochromis niloticus,
Allium test,
collagen extraction,
Comet assay,
cytotoxicity,
genotoxicity