Publicación:
Phylogeny and morphology of Hirsutella tunicata sp nov (Ophiocordycipitaceae), a novel mite parasite from Peru
Phylogeny and morphology of Hirsutella tunicata sp nov (Ophiocordycipitaceae), a novel mite parasite from Peru
No hay miniatura disponible
Fecha
2013
Autores
Ciancio, A
Colagiero, M
Rosso, LC
Gutierrez, SNM
Grasso, G
Título de la revista
Revista ISSN
Título del volumen
Editor
Elsevier
Proyectos de investigación
Unidades organizativas
Número de la revista
Abstracto
A new species of Hirsutella was isolated from unidentified mites on Petri plates inoculated
with soil and root fragments collected from asparagus rhizosphere at Viru´ , Northern Peru.
The fungus differs from other Hirsutella species by an envelope surrounding the conidium,
conidia dimension and DNA sequences. In PDA cultures, the mycelium produced aerial
hyphae with conidiogenous cells mainly at right angles, occasionally showing a secondary
conidiophore. The solitary conidia are cymbiform, slightly apiculate, 5.0e6.0 3.0e4.0 mm.
Phylogenetic analyses with partial rRNA and b-tubulin gene sequences confirmed the
fungus as an Hirsutella (Ophiocordycipitaceae). Closest species shown by maximum likelihood and neighbor-joining trees were H. nodulosa and H. aphidis, from which the new
species differs for conidium or conidiogenous cells dimensions, lack of synnemata and
host type. A recombination event was also detected in the rRNA of the holotype strain,
involving Ophiocordyceps sinensis as major parent and O. cochlidiicola as minor parent. A
complement, inverted insertion was also found in its rRNA, involving part of the ITS2 and
5.8S regions, flanked by two short nucleotide arrays. Due to conidia dimension and
phylogenetic position, the fungus is described as Hirsutella tunicata sp. nov. A review of
mononematous Hirsutella species is provided.
Descripción
The authors gratefully thank Prof. F. Porcelli (University of Bari, Italy) for accession and assistance with a light microscopy facility, Prof. S. Desantis, Dr. G. Accogli (University of Bari), Dr. G. Maglietta (Tecnopolis, Bari) for assistance with SEM and Mr. N. Salatino (IPP, CNR, Bari) for the technical assistance provided. Research partially funded by Bilateral Cooperation Agreement between Consiglio Nazionale delle Ricerche (CNR, Italy) and Consejo Nacional de Ciencia, Tecnologia e Innovacion Tecnologica (CONCYTEC, Peru) and by Project BIOMED (Ministero Politiche Agricole e Forestali, Italy).
Palabras clave
Anamorphic fungus,
Conidia,
Phylogeny,
SEM