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Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease
| dc.contributor.author | Gomes C. | es_PE |
| dc.contributor.author | Pons M.J. | es_PE |
| dc.contributor.author | Del Valle-Mendoza J. | es_PE |
| dc.contributor.author | Matsuoka M. | es_PE |
| dc.contributor.author | Ruiz J. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2021 | |
| dc.description | The study was supported by the Programa Nacional de Innovaci?n para la Competitividad y Productividad (Inn?vate Per?), under the contract 117-PNICP-PIAP-2015. J.R. had a fellowship from the program I3, of the ISCIII [grant number: CES11/012]. C.G. had a PhD fellowship of the ISCIII [FI12/00561] and was recipient of a Canon Foundation Fellowship and a Small Grant from the Royal Society for Tropical Medicine and Hygiene [grant reference 000584]. MJP had a postdoctoral fellowship from CONCYTEC/FONDECYT [grant number: CG05-2013-FONDECYT]. | |
| dc.description.abstract | Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase ? (SCS-?) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-? was determined byWestern blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-? (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-?. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-?-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-?-12 differentiating Bartonella spp. from other microorganisms. © 2021 by the authors. | |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.doi | https://doi.org/10.3390/pathogens10080917 | |
| dc.identifier.scopus | 2-s2.0-85111660194 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/3012 | |
| dc.language.iso | eng | |
| dc.publisher | MDPI | |
| dc.relation.ispartof | Pathogens | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Peptides | |
| dc.subject | Bartonella bacilliformis | es_PE |
| dc.subject | Carrion’s disease | es_PE |
| dc.subject | Diagnostic | es_PE |
| dc.subject | ELISA | es_PE |
| dc.subject | Immunogenic tools | es_PE |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.03.08 | |
| dc.title | Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication |