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Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease
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Fecha
2021
Autores
Gomes C.
Pons M.J.
Del Valle-Mendoza J.
Matsuoka M.
Ruiz J.
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MDPI
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Abstracto
Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase ? (SCS-?) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-? was determined byWestern blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-? (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-?. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-?-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-?-12 differentiating Bartonella spp. from other microorganisms. © 2021 by the authors.
Descripción
The study was supported by the Programa Nacional de Innovaci?n para la Competitividad y Productividad (Inn?vate Per?), under the contract 117-PNICP-PIAP-2015. J.R. had a fellowship from the program I3, of the ISCIII [grant number: CES11/012]. C.G. had a PhD fellowship of the ISCIII [FI12/00561] and was recipient of a Canon Foundation Fellowship and a Small Grant from the Royal Society for Tropical Medicine and Hygiene [grant reference 000584]. MJP had a postdoctoral fellowship from CONCYTEC/FONDECYT [grant number: CG05-2013-FONDECYT].
Palabras clave
Peptides,
Bartonella bacilliformis,
Carrion’s disease,
Diagnostic,
ELISA,
Immunogenic tools