Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease

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Gomes C.
Pons M.J.
Del Valle-Mendoza J.
Matsuoka M.
Ruiz J.
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Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase ? (SCS-?) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-? was determined byWestern blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-? (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-?. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-?-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-?-12 differentiating Bartonella spp. from other microorganisms. © 2021 by the authors.
The study was supported by the Programa Nacional de Innovaci?n para la Competitividad y Productividad (Inn?vate Per?), under the contract 117-PNICP-PIAP-2015. J.R. had a fellowship from the program I3, of the ISCIII [grant number: CES11/012]. C.G. had a PhD fellowship of the ISCIII [FI12/00561] and was recipient of a Canon Foundation Fellowship and a Small Grant from the Royal Society for Tropical Medicine and Hygiene [grant reference 000584]. MJP had a postdoctoral fellowship from CONCYTEC/FONDECYT [grant number: CG05-2013-FONDECYT].
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Peptides, Bartonella bacilliformis, Carrion’s disease, Diagnostic, ELISA, Immunogenic tools