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The immunoglobulin M-Shed acute phase antigen (SAPA)-test for the early diagnosis of congenital Chagas disease in the time of the elimination goal of mother-to-child transmission
The immunoglobulin M-Shed acute phase antigen (SAPA)-test for the early diagnosis of congenital Chagas disease in the time of the elimination goal of mother-to-child transmission
| dc.contributor.author | Castro-Sesquen Y.E. | es_PE |
| dc.contributor.author | Tinajeros F. | es_PE |
| dc.contributor.author | Bern C. | es_PE |
| dc.contributor.author | Galdos-Cardenas G. | es_PE |
| dc.contributor.author | Malaga E.S. | es_PE |
| dc.contributor.author | Valencia Ayala E. | es_PE |
| dc.contributor.author | Hjerrild K. | es_PE |
| dc.contributor.author | Clipman S.J. | es_PE |
| dc.contributor.author | Lescano A.G. | es_PE |
| dc.contributor.author | Bayangos T. | es_PE |
| dc.contributor.author | Castillo W. | es_PE |
| dc.contributor.author | Menduiña M.C. | es_PE |
| dc.contributor.author | Talaat K.R. | es_PE |
| dc.contributor.author | Gilman R.H. | es_PE |
| dc.contributor.author | Verastegui M. | es_PE |
| dc.contributor.author | Calderon M. | es_PE |
| dc.contributor.author | Chávez C. | es_PE |
| dc.contributor.author | Leigue J.K. | es_PE |
| dc.contributor.author | Hinojosa E. | es_PE |
| dc.contributor.author | Urquizu F. | es_PE |
| dc.contributor.author | Gorena M. | es_PE |
| dc.contributor.author | Serrudo V. | es_PE |
| dc.contributor.author | Cabrera L. | es_PE |
| dc.contributor.author | Romero Y.K. | es_PE |
| dc.contributor.author | Chagas Working Group in Bolivia and Peru | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2021 | |
| dc.description | The work was supported by Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica, FONDECYT, Peru (N084-2016) to Y. E. C.-S.; the National Institutes of Health (R01-AI87776 and D43-TW010074 to R. H. G. and D43 TW007393 to A. G. L.); and InBios International, Inc to R. H. G. through the Johns Hopkins Bloomberg School of Public Health. | |
| dc.description.abstract | Background: Diagnosis of congenital Chagas disease (CChD) in most endemic areas is based on low-sensitive microscopy at birth and 9-month immunoglobulin G (IgG), which has poor adherence. We aim to evaluate the accuracy of the Immunoglobulin M (IgM)-Shed Acute Phase Antigen (SAPA) test in the diagnosis of CChD at birth. Methods: Two cohort studies (training and validation cohorts) were conducted in 3 hospitals in the department of Santa Cruz, Bolivia. Pregnant women were screened for Chagas disease, and all infants born to seropositive mothers were followed for up to 9 months to diagnose CChD. A composite reference standard was used to determine congenital infection and was based on the parallel use of microscopy, quantitative polymerase chain reaction (qPCR), and IgM-trypomastigote excreted-secreted antigen (TESA) blot at birth and/or 1 month, and/or the detection of anti-Trypanosoma cruzi IgG at 6 or 9 months. The diagnostic accuracy of the IgM-SAPA test was calculated at birth against the composite reference standard. Results: Adherence to the 6- or 9-month follow-up ranged from 25.3% to 59.7%. Most cases of CChD (training and validation cohort: 76.5% and 83.7%, respectively) were detected during the first month of life using the combination of microscopy, qPCR, and/or IgM-TESA blot. Results from the validation cohort showed that when only 1 infant sample obtained at birth was evaluated, the qPCR and the IgM-SAPA test have similar accuracy (sensitivity: range, 79.1%-97.1% and 76.7%-94.3%, respectively, and specificity: 99.5% and 92.6%, respectively). Conclusions: The IgM-SAPA test has the potential to be implemented as an early diagnostic tool in areas that currently rely only on microscopy. © 2020 The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. | |
| dc.description.sponsorship | Fondo Nacional de Desarrollo Científico y Tecnológico - Fondecyt | |
| dc.identifier.doi | https://doi.org/10.1093/cid/ciaa986 | |
| dc.identifier.scopus | 2-s2.0-85112125413 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/3063 | |
| dc.language.iso | eng | |
| dc.publisher | Oxford University Press | |
| dc.relation.ispartof | Clinical Infectious Diseases | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Trypanosoma cruzi | |
| dc.subject | Congenital Chagas disease | es_PE |
| dc.subject | Diagnostics | es_PE |
| dc.subject | IgM antibodies | es_PE |
| dc.subject | Shed acute-phase antigen | es_PE |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.03.08 | |
| dc.title | The immunoglobulin M-Shed acute phase antigen (SAPA)-test for the early diagnosis of congenital Chagas disease in the time of the elimination goal of mother-to-child transmission | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication | |
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