Publicación:
Study of the Acetylation Pattern of Chitosan by Pure Shift NMR

dc.contributor.author Lopez J.M. es_PE
dc.contributor.author Sánchez L.F. es_PE
dc.contributor.author Nakamatsu J. es_PE
dc.contributor.author Maruenda H. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2020
dc.description.abstract Chitosan is a biodegradable, antibacterial, and nontoxic biopolymer used in a wide range of applications including biotechnology, pharmacy, and medicine. The physicochemical and biological properties of chitosan have been associated with parameters such as the degree of polymerization (DP) and the fraction of acetylation (FA). New methods are being developed to yield chitosans of specific acetylation patterns, and, recently, a correlation between biological activity and the distribution of the acetylated units (PA: pattern of acetylation) has been demonstrated. Although there are numerous well-established methods for the determination of DP and FA values, this is not the case for PA. The methods available are either not straightforward or not sensitive enough, limiting their use for routine analysis. In this study, we demonstrate that by applying HOmodecoupled Band-Selective (HOBS) decoupling NMR on signals assigned by multidimensional Pure Shift NMR methods, PA can be easily and accurately determined on various chitosan samples. This novel methodology - easily implemented for routine analysis - could become a standard for chitosan PA assessment. In addition, by applying Spectral Aliased Pure Shift HSQC, the analysis was enhanced with the determination of triads. Copyright © 2020 American Chemical Society.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1021/acs.analchem.0c01638
dc.identifier.scopus 2-s2.0-85094956644
dc.identifier.uri https://hdl.handle.net/20.500.12390/2487
dc.language.iso eng
dc.publisher American Chemical Society
dc.relation.ispartof Analytical Chemistry
dc.rights info:eu-repo/semantics/openAccess
dc.subject Analytical Chemistry
dc.subject.ocde http://purl.org/pe-repo/ocde/ford#1.04.03
dc.title Study of the Acetylation Pattern of Chitosan by Pure Shift NMR
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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