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Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids
Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids
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Fecha
2019
Autores
Muehlig A.
Jahn I.J.
Heidler J.
Jahn M.
Weber K.
Sheen P.
Zimic M.
Cialla-May D.
Popp J.
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Editor
MDPI AG
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Abstracto
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 ?m for PZA and 21 ?m for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 ?m to 50 ?m for PZA and from 50 ?m to 80 ?m for POA. © 2019 by the authors.
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Palabras clave
Surface-enhanced Raman spectroscopy (SERS),
Drug resistant mycobacteria,
Lab-on-a-Chip (LoC),
Personal medicine,
Quantitative SERS detection of PZA and POA