Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

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Muehlig A.
Jahn I.J.
Heidler J.
Jahn M.
Weber K.
Sheen P.
Zimic M.
Cialla-May D.
Popp J.
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The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 ?m for PZA and 21 ?m for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 ?m to 50 ?m for PZA and from 50 ?m to 80 ?m for POA. © 2019 by the authors.
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Surface-enhanced Raman spectroscopy (SERS), Drug resistant mycobacteria, Lab-on-a-Chip (LoC), Personal medicine, Quantitative SERS detection of PZA and POA