Publicación:
Pepper necrotic spot virus, a new tospovirus infecting solanaceous crops in Peru

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Fecha
2012
Autores
Gonzales-Castaneda, C
Gonzales, GF
Nunez, D
Olavegoya, P
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Springer
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Abstracto
Two virus isolates, T1 and T2, causing necrotic spots on leaves and stems of pepper and tomato, respectively, were isolated in the La Joya valley, Arequipa, Peru, in 2010. These two isolates were inoculated to differential hosts for tospoviruses and showed differential fitness: T1 induced necrotic local lesions in Vigna unguiculata, whereas T2 produced only chlorotic spots. The complete nucleotide sequence of the small (S) RNA from T2 and 1863 bp of the S RNA from T1 were determined. The deduced N protein sequence showed high amino acid identity between the isolates, indicating that the T1 and T2 are isolates of the same virus. Phylogenetic analysis of both the NSs and N proteins revealed that this new tospovirus belongs to the American group. The conclusion was that this tospovirus should be considered a member of a new species.
Descripción
The authors wish to thank Dr. H. Pappu for his critical review of the manuscript and A. Redondo and I. de la Pena for technical assistance. This work was supported by Ministerio de Ciencia e Innovacion (grant RTA2009-00081), cofinanced by FEDER (European Fund for Regional Development). R. Torres was the recipient of funds from CONCYTEC (Consejo Nacional de Ciencia Tecnologia e Innovacion Tecnologica) during his stay in Madrid.
Palabras clave
Fitopatología, Ciencias de las plantas, Botánica
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