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Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system

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dc.contributor.author Sauce, Rafael es_PE
dc.contributor.author Pinto, Claudineia Aparecida Sales de Oliveira es_PE
dc.contributor.author Ayala-Jara, Carmen es_PE
dc.contributor.author Prieto Lara, Zulita Adriana es_PE
dc.contributor.author Robles Velasco, Maria Valéria es_PE
dc.contributor.author Rolim Baby, André es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2021
dc.description This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant numbers 2016/24360-4 and 2019/16169-0; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process 305250/2019-1; CONCYTEC-FONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-BM-IADT/AV); and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) Finance Code 001.
dc.description.abstract Considering the importance of the cutaneous tissue investigation and the need for the development of new protocols to non-invasively establish the safety and efficacy of dermocosmetics and topical products, we aimed at developing an HPLC-TBARS-EVSC (high performance liquid chromatography–thiobarbituric acid reactive species–ex vivo stratum corneum) assay for the lipid peroxidation measurement on subjects’ stratum corneum (SC) obtained by tape stripping; additionally, we applied the HPLC-TBARS-EVSC assay in an emulsified sunscreen system containing ethylhexyl triazone and bemotrizinol as UV filters. HPLC analysis was performed in isocratic mode with 35% methanol/65% phosphate buffer (pH 7.0) as the mobile phase. The diode detector was set at 532 nm to quantify the malondialdehyde (MDA)-TBA adduct. An ex vivo tape stripping method was applied in 10 volunteers in three pre-defined regions of the volar forearms: the control; the irradiated; and the site containing the sunscreen (2.0 mg·cm−2). Ten adhesive tapes per region were used for SC removal. An exclusive ex vivo protocol to measure SC lipid peroxidation was preliminarily developed with linearity and selectivity. The protocol suggested the use of an artificial irradiation dose (5506 KJ·m−2) to improve the assay response from the SC. The sunscreen system had a significative decrease in SC lipoperoxidative damage compared to the control. Our protocol can aid in the efficacy establishment of anti-UV and antioxidant agents, for instance, in studies that aim at elucidating the level of SC lipid peroxidation and even in carrying out baseline investigations characterizing different ethnicities and genders.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.3390/scipharm89020017
dc.identifier.scopus 2-s2.0-85106583344
dc.identifier.uri https://hdl.handle.net/20.500.12390/3045
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof Scientia Pharmaceutica
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject TBARS
dc.subject HPLC es_PE
dc.subject Stratum corneum oxidative stress es_PE
dc.subject Tape stripping es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.27
dc.title Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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