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Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca]

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dc.contributor.author Jorge Banda R. es_PE
dc.contributor.author Shirley Evangelista V. es_PE
dc.contributor.author Luis Ruiz G. es_PE
dc.contributor.author Rocío Sandoval M. es_PE
dc.contributor.author Claudia Rodríguez Ll. es_PE
dc.contributor.author Martha Valdivia C. es_PE
dc.contributor.author Alexei Santiani A. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2009
dc.description Se agradece al Consejo Nacional de Ciencia y Tecnología (CONCYTEC) por el financiamiento del presente trabajo a través del Proyecto PROCYT N.°157-2006 «Desarrollo de un protocolo de criopreservación de semen de alpacas mediante el estudio de diferentes dilutores y agentes crioprotectores sobre la funcionalidad espermática».
dc.description.abstract The objective of this study was to evaluate the effect of three semen extenders (skim milk, Tris, and Tes) on cryopreservation of epididymal alpaca sperm. Previously, the effect of timespan since slaughtering or castration to the recovery of epididymal sperm was evaluated. Twenty-four alpaca testicles were used to obtain sperm from the epididymis in a buffered saline solution (PBS). The recovery of spermatozoa was performed at 0, 35, 48, and 72 hours (6 testes per group) after slaughtering or castration. Sperm motility, concentration, and sperm membrane functional integrity were analyzed. Sperm recovered after 35 hours was not usable for cryopreservation. Sperm samples recovered at 0 hours were subjected to the process of freezing. Samples were diluted with skim milk, Tris, and Tes, cooled from 35 to 5°C in 90 minutes, packed into 0.25 ml straws and frozen in liquid nitrogen. After thawing, straws were evaluated by motility, sperm membrane functional integrity and vitality/acrosome integrity. Motility was 17.0, 14.0, and 8.6% on skim milk, Tris and Tes groups respectively, where the skim milk group was significantly better than the Tes (p<0.05). Percentages of sperm membrane functional integrity and vitality/acrosomal integrity were similar among the three extenders. It was concluded that all three extenders provided similar effects for the cryopreservation of epididymal alpaca spermatozoa.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.scopus 2-s2.0-85047112658
dc.identifier.uri https://hdl.handle.net/20.500.12390/895
dc.language.iso spa
dc.publisher Universidad Nacional Mayor de San Marcos
dc.relation.ispartof Revista de Investigaciones Veterinarias del Peru
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject espermatozoides
dc.subject epididimarios es_PE
dc.subject dilutores es_PE
dc.subject criopreservación de semen es_PE
dc.subject alpaca es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.07
dc.title Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca]
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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