Publicación:
A Complementary Mechanism of Bacterial mRNA Translation Inhibition by Tetracyclines

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Fecha
2021
Autores
Das J.
Idh N.
Karlsson L.
Lerm M.
Méndez-Aranda M.
Nilsson M.
Paues J.
Pehrson I.
Sayyab S.
Tyrén A.
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Frontiers Media S.A.
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Abstracto
Tetracycline has positively impacted human health as well as the farming and animal industries. Its extensive usage and versatility led to the spread of resistance mechanisms followed by the development of new variants of the antibiotic. Tetracyclines inhibit bacterial growth by impeding the binding of elongator tRNAs to the ribosome. However, a small number of reports indicated that Tetracyclines could also inhibit translation initiation, yet the molecular mechanism remained unknown. Here, we use biochemical and computational methods to study how Oxytetracycline (Otc), Demeclocycline (Dem), and Tigecycline (Tig) affect the translation initiation phase of protein synthesis. Our results show that all three Tetracyclines induce Initiation Factor IF3 to adopt a compact conformation on the 30S ribosomal subunit, similar to that induced by Initiation Factor IF1. This compaction was faster for Tig than Dem or Otc. Furthermore, all three tested tetracyclines affected IF1-bound 30S complexes. The dissociation rate constant of IF1 in early 30S complexes was 14-fold slower for Tig than Dem or Otc. Late 30S initiation complexes (30S pre-IC or IC) exhibited greater IF1 stabilization by Tig than for Dem and Otc. Tig and Otc delayed 50S joining to 30S initiation complexes (30S ICs). Remarkably, the presence of Tig considerably slowed the progression to translation elongation and retained IF1 in the resulting 70S initiation complex (70S IC). Molecular modeling of Tetracyclines bound to the 30S pre-IC and 30S IC indicated that the antibiotics binding site topography fluctuates along the initiation pathway. Mainly, 30S complexes show potential contacts between Dem or Tig with IF1, providing a structural rationale for the enhanced affinity of the antibiotics in the presence of the factor. Altogether, our data indicate that Tetracyclines inhibit translation initiation by allosterically perturbing the IF3 layout on the 30S, retaining IF1 during 70S IC formation, and slowing the transition toward translation elongation. Thus, this study describes a new complementary mechanism by which Tetracyclines may inhibit bacterial protein synthesis. © Copyright © 2021 Barrenechea, Vargas-Reyes, Quiliano and Milón.
Descripción
We are very thankful to Marcos Milla for donating equipment that was used in this study and others. We particularly thank Andrey Konevega and his team for providing the tRNAs that were used in this study. Also, we would like to thank all lab members of the Mil?n and Quiliano groups for their help, support, and fantastic working atmosphere. Funding. This work was supported by grants from the Peruvian Fondo Nacional de Desarrollo Cient?fico, Tecnol?gico y de Innovaci?n Tecnol?gica (154-2017-FONDECYT) and (036-2019-FONDECYT-BM-INC.INV) to PM and (406-2019-FONDECYT) to MQ and the Inn?vate Per? program (297-InnovatePeru-2016) to PM. Funding for open access was provided by the Universidad Peruana de Ciencias Aplicadas (C-014-2020).
Palabras clave
translation initiation, antibiotic, initiation factor, ribosome, tetracycline, tigecycline
Citación