Publicación:
Initial intracellular proteome profile of Aspergillus Niger biofilms
Initial intracellular proteome profile of Aspergillus Niger biofilms
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Fecha
2009
Autores
Villena G.K.
Venkatesh L.
Yamazaki A.
Tsuyumu S.
Gutiérrez-Correa M.
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Facultad de Ciencias Biologicas, Universidad Nacional Mayor de San Marcos
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Abstracto
An initial profiling of the intracellular proteome of Aspergillus niger ATCC 10864 biofilm cultures developed onpolyester cloth was carried out by using 2D-PAGE and MS-TOF analysis and it was compared to the proteomeof conventionally grown free-living submerged cultures. A number of 2D-PAGE protein spots from both typesof cultures were subjected to MS-TOF analysis and data interrogation of the NCBI nr database available forthis species. Proteomic maps showed different expression patterns in both culture systems with differentiallyexpressed proteins in each case. In biofilm cultures, 19% and 32% of the selected protein spots were overexpressed and differentially expressed, respectively. On the contrary, in free-living cultures, 44% and 7% of theselected protein spots were over-expressed and differentially expressed, respectively. Although preliminary,results presented in this paper show that there are significant differences between the proteomes of A. nigerbiofilm and free-living mycelia. It seems that cell adhesion is the most important stimulus responsible for biofilmdevelopment which is the basis of Surface Adhesion Fermentation.
Descripción
This work was supported by INCAGRO (Ministry of Agriculture, Perú), CONCYTEC (Ministry of Education, Perú), and the Institute of Molecular Biology and Biotechnology (Shizuoka Univesity, Japan).
Palabras clave
proteome,
Asperigillus niger,
biofilms