Publicación:
Microbial characterization of a natural biofilm associated with peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
Microbial characterization of a natural biofilm associated with peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
| dc.contributor.author | González-Pazmiño J. | es_PE |
| dc.contributor.author | Pretell K.M. | es_PE |
| dc.contributor.author | Zapata-Vidaurre K. | es_PE |
| dc.contributor.author | Mesones M.L. | es_PE |
| dc.contributor.author | Quimí-Mujica J. | es_PE |
| dc.contributor.author | Diringer B. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Biofilms strongly influence bivalve settlement patterns on artificial substrates; however, their structure and taxonomic composition remains a black box. We characterized a natural biofilm composition that exhibits a large settlement of larvae of the Peruvian scallop Argopecten purpuratus by culture-dependent and culture-independent methods. Thirty-two different strains, representing six genera (10 strains of Bacillus, 9 of Vibrio, 6 Acinetobacter, 4 Staphylococcus, 2 Photobacterium, and 1 Exiguobacterium) were isolated. Those strains represented only 1.09% of the relative abundance compared with the total microbiota obtained by 16S rRNA high-throughput sequencing. The metagenomic analysis identified 441 species. Prokaryotes were predominant (93.4%) over eukaryotes (6.6%), with Pelobacter (13.4%), Lewinella (5.6%), Marinobacter (5.4%), Hoeflea (4.2%), and Microcystis (3.1%) being the most representative genera. Laser scanning confocal microscopy (LSCM) imaging evidenced an irregular and heterogeneous biofilm with an average thickness of 35 µm, where the heterotrophic prokaryotic community (3.4×106 cell cm-2) dominate the photoautotrophic communities (2.3×105 cell cm-2). For the first time, an A. purpuratus settlement-related biofilm was described by the next generation sequencing tool (NGS) and compared with traditional methodologies. | |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.doi | https://doi.org/10.3856/vol49-issue1-fulltext-2547 | |
| dc.identifier.scopus | 2-s2.0-85103860313 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/2425 | |
| dc.language.iso | eng | |
| dc.publisher | Escuela de Ciencias del Mar | |
| dc.relation.ispartof | urn:issn:0718560X | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Settlement | |
| dc.subject | Argopecten purpuratus | es_PE |
| dc.subject | Biofilm | es_PE |
| dc.subject | Larvae | es_PE |
| dc.subject | LSCM | es_PE |
| dc.subject | Microbiota | es_PE |
| dc.subject | NGS | es_PE |
| dc.subject.ocde | http://purl.org/pe-repo/ocde/ford#1.06.01 | |
| dc.title | Microbial characterization of a natural biofilm associated with peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication |