Publicación:
Production and characterization of a highly pure RNA polymerase holoenzyme from Mycobacterium tuberculosis
Production and characterization of a highly pure RNA polymerase holoenzyme from Mycobacterium tuberculosis
| dc.contributor.author | Herrera-Asmat O. | es_PE |
| dc.contributor.author | Lubkowska L. | es_PE |
| dc.contributor.author | Kashlev M. | es_PE |
| dc.contributor.author | Bustamante C.J. | es_PE |
| dc.contributor.author | Guerra D.G. | es_PE |
| dc.contributor.author | Kireeva M.L. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Recent publications have shown that active RNA polymerase (RNAP) from Mycobacterium tuberculosis (MtbRNAP) can be produced by expressing all four subunits in a single recombinant Escherichia coli strain [1–3]. By reducing the number of plasmids and changing the codon usage of the Mtb genes in the co-expression system published by Banerjee et al. [1], we present a simplified, detailed and reproducible protocol for the purification of recombinant MtbRNAP containing the ω subunit. Moreover, we describe the formation of ternary elongation complexes (TECs) with a short fluorescence-labeled RNA primer and DNA oligonucleotides, suitable for transcription elongation studies. | |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.doi | https://doi.org/10.1016/j.pep.2017.03.013 | |
| dc.identifier.scopus | 2-s2.0-85016006342 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/751 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Protein Expression and Purification | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | recombinant protein | |
| dc.subject | bacterial protein | es_PE |
| dc.subject | DNA directed RNA polymerase | es_PE |
| dc.subject | holoenzyme | es_PE |
| dc.subject | biosynthesis | es_PE |
| dc.subject | chemistry | es_PE |
| dc.subject | enzymology | es_PE |
| dc.subject | Escherichia coli | es_PE |
| dc.subject | genetic transcription | es_PE |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.00.00 | |
| dc.title | Production and characterization of a highly pure RNA polymerase holoenzyme from Mycobacterium tuberculosis | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# |