Publicación:
Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children

dc.contributor.author Martínez-Puchol S. es_PE
dc.contributor.author Riveros M. es_PE
dc.contributor.author Ruidias K. es_PE
dc.contributor.author Granda A. es_PE
dc.contributor.author Ruiz-Roldán L. es_PE
dc.contributor.author Zapata-Cachay C. es_PE
dc.contributor.author Ochoa T.J. es_PE
dc.contributor.author Pons M.J. es_PE
dc.contributor.author Ruiz J. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2021
dc.description.abstract The objective of the present study was to characterize Salmonella enterica serovar Infantis isolated from chicken meat determining their clonal relationships with S. Infantis isolated from children with diarrhea. Fifteen meat-recovered S. Infantis were analyzed. Susceptibility levels to 14 antibacterial agents, the presence of ESBL and that of inducible plasmid-mediated AmpC (i-pAmpC) were determined by phenotypical methods. The presence of ESBL and pAmpC was confirmed by PCR, and detected ESBL-encoding genes were sequenced and their transferability tested by conjugation. The presence of gyrA mutations as well as Class 1 integrons was determined by PCR. Clonal relationships were established by REP-PCR and RAPD. In addition, 25 clinical isolates of S. Infantis were included in clonality studies. All meat-recovered S. Infantis were MDR, showing resistance to ampicillin, nitrofurans and quinolones, while none was resistant to azithromycin, ceftazidime or imipenem. ESBL (blaCTX-M-65) and i-pAmpC (blaDHA) were detected in 2 and 5 isolates respectively (in one case concomitantly), with blaCTX-M-65 being transferable through conjugation. In addition, 1 isolate presented a blaSHV gene. All isolates presented D87Y at GyrA, nalidixic acid active efflux pump and a Class 1 integron of ~1000 bp (aadA1). Clonal analysis showed that all isolates were related. Further they were identical to MDR blaCTX-M-65-producing S. Infantis isolates causing children diarrhea in Lima. The dissemination of MDR blaCTX-M-65-producing S. Infantis between marketed meat and children highlights a public health problem which needs be controlled at livestock level. © 2021 Elsevier B.V.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1016/j.ijfoodmicro.2021.109109
dc.identifier.scopus 2-s2.0-85101925144
dc.identifier.uri https://hdl.handle.net/20.500.12390/2342
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof International Journal of Food Microbiology
dc.rights info:eu-repo/semantics/openAccess
dc.subject Foodborne diseases
dc.subject Antibiotic resistance es_PE
dc.subject ESBL es_PE
dc.subject.ocde http://purl.org/pe-repo/ocde/ford#3.03.04
dc.title Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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