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Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines

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dc.contributor.author Enciso-Benavides J. es_PE
dc.contributor.author Alfaro L. es_PE
dc.contributor.author Castañeda-Altamirano C. es_PE
dc.contributor.author Rojas, N. es_PE
dc.contributor.author González-Cabeza J. es_PE
dc.contributor.author Enciso N. es_PE
dc.contributor.author Riesco F. es_PE
dc.contributor.author Castillo M. es_PE
dc.contributor.author Enciso J. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2021
dc.description This work was supported by Proyecto CONCYTEC–Banco Mundial “Mejoramiento y Ampliación de los Servicios del Sistema Nacional de Ciencia Tecnología e Innovación Tecnológica” 8682-PE ( 104-2018-FONDECYT-BM-IADT-AV ).
dc.description.abstract Triple-negative breast tumours (TNBTs) make up 15–20% of all breast tumours. There is no treatment for them, and the role that cancer stem cells (CSCs) have in carcinogenesis is still unclear, so finding markers and therapeutic targets in CSC exosomes requires these cells to exist as a homogeneous cell population. The objective of this work was to determine differences in ultrastructural morphology, proliferative capacity, and mouse-xenotransplantation characteristics of the MDA-MB-231 and MDA-MB-436 TNBT cell lines with the CD44high/CD24low phenotype in order to study their exosomes. The results show that the CD44high/CD24low MBA-MB-231 cells had a population doubling time of 41.56 h, compared to 44.79 h in the MDA-MB-436 cell line. After magnetic immunoseparation, 18.75% and 14.56% of the stem cell population of the MDA-MB-231 and MDA-MB-436 cell lines, respectively, were of the CD44high/CD24low phenotype, which were expanded to reach purities of 80.4% and 87.6%. The same expanded lineage in both cell lines was shown to possess the pluripotency markers Nanog and Oct4. Under a scanning electron microscope, the CD44high/CD24low lineage of the MBA-MD-231 cell line formed groups of more interconnected cells than this lineage of the MBA-MD-436 line. A total of 16% of the mice inoculated with the CD44high/CD24low lineage of either cell line presented tumours of the breast, lung, and submandibular ganglia, in whose tissues variable numbers of inoculated cells were found 30 days post-inoculation. By magnetic immunoselection, it was possible to isolate in similar quantities and characterize, expand, and xenotransplant the CD44high/CD24low lineage of the MDA-MB-231 and MDA-MB-436 cell lines. The former cell line has greater proliferative capacity, the two lines differ under scanning electron microscopy in how they intercommunicate, and both cell lines induce new tumours in mice and persist at least 30 days post-inoculation in the transplanted animal so their exosomes would also be different. © 2021 The Author(s)
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1016/j.heliyon.2021.e07273
dc.identifier.scopus 2-s2.0-85109559504
dc.identifier.uri https://hdl.handle.net/20.500.12390/2977
dc.language.iso eng
dc.publisher Elsevier Ltd
dc.relation.ispartof Heliyon
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject Triple-negative breast tumors
dc.subject Cancer stem cells es_PE
dc.subject CD44high/CD24low phenotype cells es_PE
dc.subject Magnetic immunoselection es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.04.02
dc.title Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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