Antimicrobial peptides purified from hydrolysates of kanihua (Chenopodium pallidicaule Aellen) seed protein fractions

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Moscoso-Mujica, Gladys
Zavaleta, Amparo, I
Mujica, Angel
Arnao, Ines
Moscoso-Neira, Carlos
Santos, Marco
Sanchez, Johanna
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Elsevier Ltd
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The kanihua (Chenopodium pallidicaule Aellen) Andean grain from the Peruvian Altiplano presents proteins of 15% to 19%. The objective was to obtain purified bioactive antimicrobial peptides (AMPs), hydrolyzed with Alcalase and Pepsin-pancreatin sequential system of protein fractions of kanihua varieties Ramis (KR) and CupiSayhua (KS), and hydrolysates with different degrees of hydrolysis (DH) and percentage inhibition (IP) of the growth of E. coli, S. aureus, and C. albicans. To obtain AMPs, nutraceuticals, bio-preservatives, and novel ingredients in food design. The results showed 216 hydrolysates (1%, w/v), only 28 presented significant difference compared to controls (IP 0.05), and presented an anionic charge. In conclusion, the simulated digestion in vitro showed higher DH (7%-67%) than Alcalase (13%-54%); the majority were extensive; of 28 hydrolysates with IP 45% 4 AMPs with important IPs were obtained, and one was anionic.
CIENCIACTIVA-CONCYTEC funded this research under contract 007-2014-FONDECYT. The professional collaboration and unconditional support of the Business administrator Pawel Moscoso-Mujica in carrying out the research are appreciated.
Palabras clave
Protein fractions, Chenopodium pallidicaule Aellen, Antimicrobial peptides, Hydrolyzed, Antimicrobial activity in percent inhibition, Degree of hydrolysis, Nutraceuticals