Publicación:
Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru

dc.contributor.author Murray M. es_PE
dc.contributor.author Salvatierra G. es_PE
dc.contributor.author Dávila-Barclay A. es_PE
dc.contributor.author Ayzanoa B. es_PE
dc.contributor.author Castillo-Vilcahuaman C. es_PE
dc.contributor.author Huang M. es_PE
dc.contributor.author Pajuelo M.J. es_PE
dc.contributor.author Lescano A.G. es_PE
dc.contributor.author Cabrera L. es_PE
dc.contributor.author Calderón M. es_PE
dc.contributor.author Berg D.E. es_PE
dc.contributor.author Gilman R.H. es_PE
dc.contributor.author Tsukayama P. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2021
dc.description.abstract The widespread and poorly regulated use of antibiotics in animal production in low- and middle-income countries (LMICs) is increasingly associated with the emergence and dissemination of antibiotic resistance genes (ARGs) in retail animal products. Here, we compared Escherichia coli from chickens and humans with varying levels of exposure to chicken meat in a low-income community in the southern outskirts of Lima, Peru. We hypothesize that current practices in local poultry production result in highly resistant commensal bacteria in chickens that can potentially colonize the human gut. E. coli was isolated from cloacal swabs of non-organic (n = 41) and organic chickens (n = 20), as well as from stools of market chicken vendors (n = 23), non-vendors (n = 48), and babies (n = 60). 315 E. coli isolates from humans (n = 150) and chickens (n = 165) were identified, with chickens showing higher rates of multidrug-resistant and extended-spectrum beta-lactamase phenotypes. Non-organic chicken isolates were more resistant to most antibiotics tested than human isolates, while organic chicken isolates were susceptible to most antibiotics. Whole-genome sequencing of 118 isolates identified shared phylogroups between human and animal populations and 604 ARG hits across genomes. Resistance to florfenicol (an antibiotic commonly used as a growth promoter in poultry but not approved for human use) was higher in chicken vendors compared to other human groups. Isolates from non-organic chickens contained genes conferring resistance to clinically relevant antibiotics, including mcr-1 for colistin resistance, blaCTX-M ESBLs, and blaKPC-3 carbapenemase. Our findings suggest that E. coli strains from market chickens are a potential source of ARGs that can be transmitted to human commensals. © Copyright © 2021 Murray, Salvatierra, Dávila-Barclay, Ayzanoa, Castillo-Vilcahuaman, Huang, Pajuelo, Lescano, Cabrera, Calderón, Berg, Gilman and Tsukayama.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.3389/fmicb.2021.635871
dc.identifier.scopus 2-s2.0-85102799144
dc.identifier.uri https://hdl.handle.net/20.500.12390/2374
dc.language.iso eng
dc.publisher Frontiers Media S.A.
dc.relation.ispartof Frontiers in Microbiology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject WGS
dc.subject AMR es_PE
dc.subject Escherichia coli es_PE
dc.subject genomics es_PE
dc.subject LMIC es_PE
dc.subject one health es_PE
dc.subject Peru es_PE
dc.subject poultry es_PE
dc.subject.ocde http://purl.org/pe-repo/ocde/ford#4.02.02
dc.title Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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