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Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru

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dc.contributor.author Coronado-Olano J. es_PE
dc.contributor.author Repo-Carrasco-Valencia R. es_PE
dc.contributor.author Reategui O. es_PE
dc.contributor.author Toscano E. es_PE
dc.contributor.author Valdez E. es_PE
dc.contributor.author Zimic M. es_PE
dc.contributor.author Best I. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2021
dc.description This study was funded by a grant from the National Fund for Scientific, Technological Development and Technological Innovation (FONDECYT) of the National Council of Science, Technology and Technological Innovation (CONCYTEC) of Peru, Contract N° 171-2015-FONDECYT.
dc.description.abstract Background: Chenopodium quinoa Willd. and Chenopodium pallidicaule Aellen are grains from the Andean region of Peru, which in addition to having a high nutritional value, have health-promoting properties related to the prevention of chronic diseases such as diabetes. Objetive: The present study aimed to identify phenolic compounds associated with an inhibition of carbohydrate hydrolyzing enzymes associated with type 2 diabetes. Material and Methods: Two varieties of quinoa and two varieties of cañihua from the Puno Region in Peru, were evaluated. Total phenolics, total flavonoids, identification of phenolic compounds and antioxidant activity in vitro were measured by Folin-Ciocalteu assay, aluminum chloride colorimetric method, HPLC-DAD and DPPH radical scavenging assay, respectively. In vitro hypoglycemic activity was evaluated through the inhibition of the ?-amylase and ?-glucosidase enzymes. Results: Gallic acid, rutin and chlorogenic acid were identified by HPLC-DAD in the varieties of quinoa and cañihua. The latter showed significantly higher levels of chlorogenic acid compared to quinoa varieties (p<0.05). Both Andean grains exhibited inhibition of key-enzymes linked to type 2 diabetes, presenting IC50 values of 7.99 to 34.05 and of 8.07 to 1158 µg/mL for ?-amylase and ?-glucosidase, respectively. Total phenolics, total flavonoids, DPPH radical scavenging assay, gallic acid and chlorogenic acid showed the greatest contribution to the inhibitory activity of the ?-glucosidase enzyme (p<0.05). Conclusion: Our findings suggest that the phenolic compounds present in the varieties of quinoa and cañihua could modulate the inhibition of carbohydrate hydrolyzing enzymes associated with type 2 diabetes. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.5530/pj.2021.13.115
dc.identifier.scopus 2-s2.0-85110944883
dc.identifier.uri https://hdl.handle.net/20.500.12390/3013
dc.language.iso eng
dc.publisher EManuscript Technologies
dc.relation.ispartof Pharmacognosy Journal
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject Polyphenols
dc.subject Andean grains es_PE
dc.subject DPPH radical scavenging assay es_PE
dc.subject HPLC-DAD es_PE
dc.subject In vitro antidiabetic activity es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03
dc.title Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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