Publicación:
Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
No hay miniatura disponible
Fecha
2013
Autores
Vásquez J.
Florentini E.A.
Camargo L.A.
Gonzales J.
Valdivia M.
Título de la revista
Revista ISSN
Título del volumen
Editor
Elsevier
Proyectos de investigación
Unidades organizativas
Número de la revista
Abstracto
The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15 min exposure time could generate the same results as longer incubation times (30, 45 and 60 min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300 mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37 °C. According to the percentage of reacted spermatozoa, 75–100 mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15 min to be considered sufficient incubation time.
Descripción
This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .
Palabras clave
Zoología,
Genética animal