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Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging

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dc.contributor.author Iglesias-Pedraz J.M. es_PE
dc.contributor.author Comai L. es_PE
dc.date.accessioned 2024-05-30T23:13:38Z
dc.date.available 2024-05-30T23:13:38Z
dc.date.issued 2020
dc.description.abstract Reactive oxygen species (ROS) represent a number of highly reactive oxygen-derived by-products generated by the normal mitochondrial respiration and other cellular metabolic reactions. ROS can oxidize macromolecules including lipids, proteins, and nucleic acids. Under physiological condition, the cellular levels of ROS are controlled by several antioxidant enzymes. However, an imbalance between ROS production and detoxification results in oxidative stress, which leads to the accumulation of macromolecular damage and progressive decline in normal physiological functions. Oxidative deterioration of DNA can result in lesion that are mutagenic and contribute to aging and age-related diseases. Therefore, methods for the detection of ROS and oxidative deterioration of macromolecules such as DNA in cells provide important tool in aging research. Here, we described protocols for the detection of cytoplasmic and mitochondria pools of hydrogen peroxide, and the DNA modification 8-oxoguanine, a biomarker of oxidative damage, that are applicable to cell-based studies on aging and other related areas. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
dc.description.sponsorship Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec
dc.identifier.doi https://doi.org/10.1007/978-1-0716-0592-9_22
dc.identifier.scopus 2-s2.0-85085157384
dc.identifier.uri https://hdl.handle.net/20.500.12390/2645
dc.language.iso eng
dc.publisher Humana Press Inc.
dc.relation.ispartof Methods in Molecular Biology
dc.rights info:eu-repo/semantics/openAccess
dc.subject Senescence
dc.subject 8-Oxoguanine es_PE
dc.subject Cancer es_PE
dc.subject DNA damage es_PE
dc.subject Flow cytometry es_PE
dc.subject Hydrogen peroxide es_PE
dc.subject Immunofluorescence es_PE
dc.subject MitoPY-1 es_PE
dc.subject Oxidative stress es_PE
dc.subject PF6-AM es_PE
dc.subject.ocde http://purl.org/pe-repo/ocde/ford#3.04.03
dc.title Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging
dc.type info:eu-repo/semantics/article
dspace.entity.type Publication
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