Bienvenido al Repositorio Institucional del Concytec

El Repositorio Institucional del Concytec tiene como objetivo permitir el libre acceso a la producción científica institucional, optimizando su visibilidad; así mismo garantizar la preservación y conservación de la información relacionada a la ciencia, tecnología e innovación.

Display.item.jsp


Por favor, utiliza este identificador para citar o enlazar este ítem:
http://hdl.handle.net/20.500.12390/2840


Título: WRN modulates translation by influencing nuclear mRNA export in HeLa cancer cells
Autor(es): Manuel Iglesias-Pedraz, Juan 
Matia Fossatti-Jara, Diego 
Valle-Riestra-Felice, Valeria 
Rafael Cruz-Visalaya, Sergio 
Felix, Jose Antonio Ayala 
Comai, Lucio 
Resumen: Background The Werner syndrome protein (WRN) belongs to the RecQ family of helicases and its loss of function results in the premature aging disease Werner syndrome (WS). We previously demonstrated that an early cellular change induced by WRN depletion is a posttranscriptional decrease in the levels of enzymes involved in metabolic pathways that control macromolecular synthesis and protect from oxidative stress. This metabolic shift is tolerated by normal cells but causes mitochondria dysfunction and acute oxidative stress in rapidly growing cancer cells, thereby suppressing their proliferation. Results To identify the mechanism underlying this metabolic shift, we examined global protein synthesis and mRNA nucleocytoplasmic distribution after WRN knockdown. We determined that WRN depletion in HeLa cells attenuates global protein synthesis without affecting the level of key components of the mRNA export machinery. We further observed that WRN depletion affects the nuclear export of mRNAs and demonstrated that WRN interacts with mRNA and the Nuclear RNA Export Factor 1 (NXF1). Conclusions Our findings suggest that WRN influences the export of mRNAs from the nucleus through its interaction with the NXF1 export receptor thereby affecting cellular proteostasis. In summary, we identified a new partner and a novel function of WRN, which is especially important for the proliferation of cancer cells.
Tema: Cell Biology;Molecular Biology
Editorial: Springer Science and Business Media LLC
Fecha de publicación: 2020
Publicado en: BMC MOLECULAR AND CELL BIOLOGY 
Financiamiento: 150-2017 
Tipo de publicación: info:eu-repo/semantics/article
Identificador Handle: http://hdl.handle.net/20.500.12390/2840
DOI: 10.1186/s12860-020-00315-9
Nivel de acceso: info:eu-repo/semantics/closedAccess
Colección:6.1 Proyectos de investigación científica

Registro Dublin Core completo



Páginas vistas

22
marcado en 17-may-2022

Google ScholarTM

Check

Altmetric

  • Compartir este item
  • QR Code

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.