Publicación:
Use of rita® temporary immersion system to obtain microtubers of several mashua Tropaeolum tuberosum Ruiz y Pavón morphotypes
Use of rita® temporary immersion system to obtain microtubers of several mashua Tropaeolum tuberosum Ruiz y Pavón morphotypes
| dc.contributor.author | Peña-Rojas G. | es_PE |
| dc.contributor.author | Carhuaz-Condori R. | es_PE |
| dc.contributor.author | Andía-Ayme V. | es_PE |
| dc.contributor.author | Dávalos-Prado J.Z. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Background. The Temporary Immersion System (TIS) is an effective strategy to obtain quality mashua microtubers through the in vitro propagation of seedlings in a small space and in a short period of time. Objective. Obtain microtubers of 27 mashua morphotypes (Tropaeolum tuberosum) using the TIS-RITA®. Methodology. The seedlings were previously propagated in MS (Murashige & Skoog) solid medium, supplemented with 3% sucrose, at 5.6 pH and 0.7% agar. After fifteen days of cultivation in this medium, the seedlings were transferred to a MS liquid. For the tuberization process, seedlings with an average size of 10 cm were transferred to TIS-RITA® systems for ten weeks, under conditions of complete darkness, at 19 ± 2 ° C, an immersion frequency of 2 minutes every 3 hours in a MS liquid medium supplemented with 8% sucrose and 2 mg.L-1 benzylamino purine (BAP). Results. The best results of microtubers were obtained with different morphotypes, which are respect to i/size, MAC 06A (1.9 cm) and MAC 056 (1.8 cm); ii / fresh weight, MAC 06A (0.2g) and MAC 092 (0.18 g); iii / quantity, MAC 08A (51 units) and MAC 048 (56 units). Implications. TIS-RITA® can be used to obtain quality and virus-free seeds of a wide variety of commercially important crops. Conclusion. The SIT-RITA® based on the intermittent contact of the explants cultivating medium, is an efficient system to obtain mashua microtubers. | |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.scopus | 2-s2.0-85099055271 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/2504 | |
| dc.language.iso | eng | |
| dc.publisher | Universidad Autonoma de Yucatan | |
| dc.relation.ispartof | Tropical and Subtropical Agroecosystems | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Tropaeolum tuberosum | |
| dc.subject | In vitro propagation | es_PE |
| dc.subject | Mashua | es_PE |
| dc.subject | Microtubers | es_PE |
| dc.subject | TIS temporary immersion system | es_PE |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.04.00 | |
| dc.title | Use of rita® temporary immersion system to obtain microtubers of several mashua Tropaeolum tuberosum Ruiz y Pavón morphotypes | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication |