Publicación:
Phenotypic and molecular differences among rhizobia that nodulate Phaseolus lunatus in the Supe valley in Peru
Phenotypic and molecular differences among rhizobia that nodulate Phaseolus lunatus in the Supe valley in Peru
No hay miniatura disponible
Fecha
2015
Autores
Matsubara, M
Zuniga-Davila, D
Título de la revista
Revista ISSN
Título del volumen
Editor
Springer Nature
Proyectos de investigación
Unidades organizativas
Número de la revista
Abstracto
A collection of 16 bacterial strains isolated from
root nodules of Lima bean (Phaseolus lunatus L.) in the
Supe valley of Peru were characterised using phenotypic and
molecular methods. The isolates were clustered into fastgrowing, alkalinising and extra-alkalinising slow-growing
isolates with marked morphological differences, according to
the rate of growth and alkalinisation of yeast extract mannitol
(YEM) medium. Fast-growing isolates were salt tolerant and
sensitive to 40 °C, while alkalinising slow-growing isolates
behaved oppositely. Extra-alkalinising slow-growing isolates
were sensitive to 8 °C and 40 °C and tolerated 1 % NaCl. Fastgrowing isolates also showed higher indole-3-acetic acid
(IAA) production and tri-calcium phosphate solubilisation
than the alkalinising slow-growing isolates. Half of the isolates were able to nodulate Phaseolus vulgaris and Vigna
unguiculata. Individually, BOX-PCR, ERIC-PCR, and REPPCR fingerprints patterns resulted in ten profiles from 16 isolates and they were clustered into three profile groups that
correspond to the clusters obtained by YEM medium
alkalinisation. Analysis of 16S rRNA gene sequences revealed that fast-growing isolates showed 99.7 % sequence
identity with Rhizobium mesosinicum CCBAU 25010T and
Rhizobium alamii GBV016T
. Alkalinising slow-growing isolates were related to both Bradyrhizobium yuanmingense
CCBAU10071T and Bradyrhizobium liaoningense 2281T
,
with 99.8 % sequence identity, and extra-alkalinising slowgrowing isolates had 100 % sequence identity to both
Bradyrhizobium paxllaeri LMTR 21T and Bradyrhizobium
icense LMTR 13T.
Descripción
Palabras clave
Phaseolus lunatus,
Phenotypic characterization,
Rhizobium