Publicación:
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR
| dc.contributor.author | A Castaneda C. | es_PE |
| dc.contributor.author | Castillo M. | es_PE |
| dc.contributor.author | Sanchez J. | es_PE |
| dc.contributor.author | Casavilca S. | es_PE |
| dc.contributor.author | Sanchez J. | es_PE |
| dc.contributor.author | A Bernabe L. | es_PE |
| dc.contributor.author | Suarez N. | es_PE |
| dc.contributor.author | Chavez I. | es_PE |
| dc.contributor.author | Ruiz E. | es_PE |
| dc.contributor.author | Tello K. | es_PE |
| dc.contributor.author | R Villa M. | es_PE |
| dc.contributor.author | Zevallos R. | es_PE |
| dc.contributor.author | Montenegro P. | es_PE |
| dc.contributor.author | Dias-Neto E. | es_PE |
| dc.contributor.author | Landa-Baella M. | es_PE |
| dc.contributor.author | Taxa L. | es_PE |
| dc.date.accessioned | 2024-05-30T23:13:38Z | |
| dc.date.available | 2024-05-30T23:13:38Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results. © 2020 Future Medicine Ltd.. All rights reserved. | |
| dc.description.sponsorship | Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - Concytec | |
| dc.identifier.doi | https://doi.org/10.2217/fmb-2019-0280 | |
| dc.identifier.scopus | 2-s2.0-85091266055 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12390/2514 | |
| dc.language.iso | eng | |
| dc.publisher | Future Medicine Ltd. | |
| dc.relation.ispartof | Future Microbiology | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | stomach neoplasms | |
| dc.subject | eosin | es_PE |
| dc.subject | Helicobacter pylori | es_PE |
| dc.subject | hematoxylin | es_PE |
| dc.subject | histopathology | es_PE |
| dc.subject | immunohistochemistry | es_PE |
| dc.subject | PCR | es_PE |
| dc.subject.ocde | http://purl.org/pe-repo/ocde/ford#3.04.02 | |
| dc.title | Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR | |
| dc.type | info:eu-repo/semantics/article | |
| dspace.entity.type | Publication |